Research Paper Volume 12, Issue 18 pp 17902—17920
Long noncoding RNA SBF2-AS1 contributes to the growth and metastatic phenotypes of NSCLC via regulating miR-338-3p/ADAM17 axis
Figure 6. The effect of SBF2-AS1/miR-338-3p/ADAM17 axis on NSCLC cells biological behavior in vitro. (A) SBF2-AS1, ADAM17 and miR-338-3p were co-transfected into A549 cell, the mRNA level of ADAM17 was measured by qRT-PCR assay. (B) SBF2-AS1, ADAM17 and miR-338-3p were co-transfected into A549 cell, Cell growth and invasion ability were assessed by colony formation assay and Transwell assay. (C) si-SBF2-AS1, si-ADAM17 and miR-338-3p inhibitor were co-transfected into H1975 cell, the mRNA level of ADAM17 was measured by qRT-PCR assay. (D) si-SBF2-AS1, si-ADAM17 and miR-338-3p inhibitor were co-transfected into H1975 cell, cell growth and invasion ability were assessed by colony formation assay and Transwell assay. The data are presented as the mean ± SD. All in vitro data are representative of three independent experiments.