Research Paper Volume 12, Issue 20 pp 20047—20068

Figure 2. CUEDC1 decreases the motility and invasive properties of NSCLC cells. (A) CUEDC1 protein levels in different NSCLC cell lines and normal human bronchial epithelial cell line detected by western blotting. β-actin was used as an internal control. (B) CUEDC1 expression was confirmed by immunoblotting. CUEDC1 expression in H1299 and A549 cells was reduced markedly by shRNA1 interference. (C) Over-expression of CUEDC1 in H460 cells. CUEDC1 expression was determined using western blotting. (D) Wound-healing assays were used to investigate the migration of H1299 and A549 cells. P values were calculated using Student’s t-test. (E) Migration and invasion of H1299 and A549 cells (and their derivatives) were measured using transwell assays. P values were calculated using Student’s t-test. (F) Wound-healing assays were used to examine the migration of H460 cells. P values were calculated using Student’s t-test. (G) The migration and invasion of H460 cells (and their derivatives) were performed using transwell assays. The data are expressed as the mean ± SEM. P values were calculated using Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001.