Research Paper Volume 12, Issue 13 pp 12799—12811

Figure 7. Tomatidine suppresses IL-1β-induced NF-κB activation in primary chondrocytes. (A) Representative western blot images and (B) Histogram plots show the levels of phospho-P65 and P65 proteins relative to GAPDH (internal control) levels in the primary chondrocytes treated for 30 mins with 2.5, 5, or 10μM tomatidine alone or in combination with 10 ng/mlIL-1β. DMSO was used as control. (C) Immunofluorescence staining images show P65 localization in primary chondrocytes treated for 30 mins with 2.5, 5, or 10μM tomatidine alone or in combination with 10ng/mlIL-1β. The white arrow indicates nuclear translocation of P65. The values are shown as means ± SD of triplicate experiments. #p < 0.05 compared with the control group; *p < 0.05 and **p < 0.01 compared with the IL-1β group.