Figure 1. GAS6-AS2 was highly expressed in OS tissues and associated with poor prognosis. (A) The levels of GAS6-AS2 was detected in 157 pairs of OS tissues and matched normal tissues using qRT-PCR. (B) The expression trend of GAS6-AS2 in OS patients with different clinical stages. (C) RT-PCR assays for the expressions of GAS6-AS2 in five OS cell lines and normal bone cell. (D) Kaplan-Meier curves for overall survival in patients with OS. (E) Kaplan-Meier curves for disease-free survival in patients with OS. * P < 0.05, **P < 0.01.