Figure 4.Transcription of the mutant HIF-2α protein is maintained under normoxic and hypoxic conditions. (A) Results from real-time PCR demonstrated similar transcription levels for the wildtype and mutant HIF2A genes under conditions of normoxia and simulated hypoxia. (B) A luciferase assay using Hep3B cells transfected with wildtype and p.E549K HIF2A confirmed these findings.
