Research Paper Volume 12, Issue 7 pp 5675—5692

Figure 5. DGCR8 produces a pro-growth miRNA Profile that promotes Cell Proliferation. (A) knock-down efficiency detected by qRT-PCR of transient silencing of miRNA that down-regulated in T87G cells when DGCR8 was knocked down. miR-NC, miR-scramble; Inhibitor, a contraction of miRNA inhibitor. Two-tailed, unpaired T-test was used to analyze the significant difference (miR-21-5p, t=38.44, df=4, P<0.0001; miR-34-5p, t=26.19, df=4, P<0.0001; miR-1246, t=11.66, df=4, P<0.0001; miR-4488, t=16.9, df=4, P<0.0001; miR-494, t=28.33, df=4, P<0.0001). Here *** P<0.0001 compared with control. (B) MTT assay was performed on T87G cells whose endogenous miRNAs, as shown, were transiently knocked down. Two-tailed, unpaired T-test was used to analyze the significant difference, *** P<0.001 compared with control. (C) Wound closure assay was conducted in parallel with MTT assays to appraise the migratory variation in T87G cells. Two-tailed, independent T-test was used to analyze the significant difference. (D) Transwell assays were carried out to analyze the invasive variation after differential miRNAs were transiently knocked down in T87G cells. One way ANOVA (Bonferroni approach) was taken to analyze the significant difference among groups in comparison with control group transfected with miR-NC. Here, ** P<0.001, *** P<0.0001 relative to control.