Research Paper Volume 12, Issue 7 pp 5640—5650
Inhibition of miR-19a partially reversed the resistance of colorectal cancer to oxaliplatin via PTEN/PI3K/AKT pathway
Figure 3. MiR-19a targeted PTEN in CRC. (A) Multiple regions of PTEN mRNA 3’ UTR exist potential binding sites paired with miR-19a. (B) Western blot assays were performed to test the expression of PTEN in SW480, SW480/R, HT29 and HT29/R cells. (C) Western blot assays were performed to test the effect of miR-19a (50 pmol/ml) and anti-miR-19a (50 pmol/ml) on changing the expression of PTEN in SW480, SW480/R, HT29 and HT29/R cells. (D) Luciferase reporter assays were performed to test the effect of miR-19a (50 pmol/ml) and anti-miR-19a (50 pmol/ml) on changing the luciferase activities of the pMIR reporters containing PTEN 3’-UTR in SW480, SW480/R, HT29 and HT29/R cells. *P<0.05 vs. miR-c group.