Figure 3. SIRT3 protein immunohistochemistry in rat heart tissues of NS group and ISO group. SD rats of ISO group were subjected to subcutaneous injections of 1.5 mg/kg/d isoproterenol for 7 d. The control group was given the same dose of saline. We stained SIRT3 protein with SIRT3 antibody and stained the nuclear with DAPI. Brown represents SIRT3 protein and blue represents nuclear. Representative images out of 4 independent experiments were shown.