Figure 1. Uterine type 1 collagen deposition evaluation. (A) Collagen-1 statistical Western Blot analysis, letters indicate differences between groups (P<0.05), values were plotted as mean ± standard error of the mean. (B) Collagen-1 and β-actin Western Blot bands. (C) Representative Masson’s Trichrome stained images of uterine tissue. OT: old treatment (n=4); OC: old control (n=4); YT: young treatment (n=4); YC: young control (n=4). My (OL): myometrium (outer layer); My (IL): myometrium (inner layer). Fibrosis are stained in blue.