Research Paper Volume 12, Issue 2 pp 1563—1576

Figure 2. SENP1 knockout inhibits cell proliferation and motion, and induces cell cycle dysregulation. (A) The protein levels of SENP1 in normal hepatic cells and nine HCC cell lines were measured using Western blot. (B) SENP1 knockout was confirmed by Western blot. (C) The effect of SENP1 knockout on HepG2 cell proliferation was determined using cell count assay. (D) The effects of SENP1 knockout on HepG2 cell colony formation were examined using colony formation assay. (E) The effects of SENP1 knockout on HepG2 cell cycle distribution were analyzed by using flow cytometry. (F) The effects of SENP1 knockout on P53, P21 and CyclinD1 mRNA levels were measured using RT-PCR. (G) The effects of SENP1 knockout on HepG2 cell migration were determined using wound healing assay. (H) The effects of SENP1 knockout on HepG2 cell invasion were examined using Transwell assay. The representative images were selected from at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001.