Research Paper Volume 12, Issue 2 pp 1417—1432

Figure 4. Re-activation of mitochondrial fission reverses the pro-survival effects of SRV2 knockdown in cardiomyocytes. (A) Cardiomyocyte viability was measured via MTT assay after transfection of siRNA against SRV2 and addition of FCCP to the culture medium. (B–C) TUNEL staining was used to quantify numbers of apoptotic cardiomyocytes after SRV2 siRNA transfection and FCCP treatment. (D–E) Relative expression of F-actin in cardiomyocytes was measured in an immunofluorescence assay after SRV2 siRNA transfection and FCCP treatment. (F–H) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze IL-1β, IL-8, and MCP-1 transcript levels after SRV2 siRNA transfection and FCCP treatment. *p<0.05 vs. control group, #p<0.05 vs. LPS group, @p<0.05 vs. LPS+si-SRV2 group.