Research Paper Volume 12, Issue 2 pp 1159—1170

Figure 1. H₂O₂ downregulates circHIPK3 in human osteoblasts. OB-6 human osteoblastic cells or the primary human osteoblasts were treated with hydrogen peroxide (H₂O₂, at applied concentrations) and cultured for indicated time periods, relative circHIPK3 expression was tested by qPCR (A–C) qPCR analysis of the relative circHIPK3 expression in the surgery-isolated femoral head tissues (both normal and necrotic) from ten (10) different dexamethasone-treated patients (D) “Veh” stands for vehicle control (PBS, same for all Figures). Quantified values were mean ± standard deviation (SD). * P < 0.05 vs. “Veh” treatment (A–C) * P < 0.05 vs. “S” tissues (surrounding normal femoral head tissues) (D; n=10). Experiments were repeated three times, with similar results obtained.