Figure 4. Levels of SAG and COPB2 expression in breast cancer cell lines. (A) SAG expression in human cancer cells from a cohort in TCGA database. (B) COPB2 expression in human cancer cells from a cohort in TCGA database. (C–D) RT-qPCR analysis of the relative levels of SAG expression in SKBR3 (C) and T47D (D) cells. Levels of SAG and COPB2 mRNA were significantly decreased following transfection with the indicated siRNAs in SKBR3 (E) and T47D (F) cells. (*P < 0.05).