Research Paper Volume 12, Issue 2 pp 1114—1127

Figure 5. The overexpression of Romo1 promoted macrophage polarization toward M2 phenotype. (A) The control and Romo1-overexpressed macrophages were analyzed by immunofluorescence with CD206 and iNOS antibodies. The CD206+ and iNOS+ cells were quantified and statistically analyzed. (B, C) The control and Romo1-overexpressed macrophages were analyzed by flow cytometry with M1 markers (CD11c and F4/80) and M2 markers (CD206 and CD11b). The double positive cells were respectively quantified and statistically analyzed. (D) The mRNA levels of M1-related genes (IL-6, iNOS, TNF-α) and M2-related genes (Arginase 1, Ym1, IL-10) were determined by RT-qPCR in control and Romo1-overexpressed macrophages. (E) The production of IL-1β, IL-12, TGF-β or IL-23 was respectively analyzed by ELISA in control and Romo1-overexpressed macrophages. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01.