Research Paper Volume 11, Issue 24 pp 12793—12809
Loss of Klotho contributes to cartilage damage by derepression of canonical Wnt/β-catenin signaling in osteoarthritis mice
Figure 9. In chondrocytes knockdown of β-catenin, the binding of Dvl to Frizzled, Klotho to Wnt1, Wnt4, Wnt7a and β-catenin to MMP2, 9 promoters were detected. (A) Co-IP method was used to detect the binding of Klotho and Wnt1, Wnt4, Wnt7a in different groups of cells supernatant. (B) Co-IP method was used to detect the binding of Klotho and Wnt1, Wnt4 and Wnt7a, the binding of Dvl to Frizzled in different groups of cells. quantitative data (C, D) is presented. *P,0.05 versus controls (n=3), The cell lysates were immunoprecipitated by β-catenin antibody. qPCR analyses the enrichment of MMP-2, MMP-9-specific DNA in chondrocytes. (E, F) MMP-2 and MMP-9 promoter luciferase activity decreased after silencing β-catenin. Results were obtained via the expression of three individual experiments performed in triplicate for each condition. *P < 0.05, **P < 0.01.