Research Paper Volume 11, Issue 23 pp 11722—11755

Figure 5. Fragmentation MS/MS spectra. (Panel A) Fragmentation MS/MS spectra of the modified peptide carrying the novel PTMs. MS/MS data are referred to histone H1.5. Data were analyzed by Proteome Discoverer 1.4 software. MS/MS data were searched on the Human UniProt database. False discovery rate (FDR) of peptide identifications was estimated using the “Target-decoy PSM validator” node in Proteome Discoverer. Cut off filters 95% confidence and a minimum of two peptide identifications per protein. (Panel B) Fragmentation MS/MS spectra of the modified peptide carrying the novel PTMs. MS/MS data are referred to histones H1.2 and H1.3. Data were analyzed by Proteome Discoverer 1.4 software. MS/MS data were searched on the Human UniProt database. False discovery rate (FDR) of peptide identifications was estimated using the “Target-decoy PSM validator” node in Proteome Discoverer. Cut off filters 95% confidence and a minimum of two peptide identifications per protein.