Research Paper Volume 11, Issue 23 pp 11659—11672

Figure 2. NiCl₂ activates NF-κB pathway in BMDMs. (A and B) BMDMs are treated with NiCl₂ (0, 0.1, 0.5 and 1.0 mM) for 24h, and immunoblotted for the whole cell lysis IκBα and p-IκBα protein expression. (C and D) cells are treated with NiCl₂ (0, 0.1, 0.5 and 1.0 mM) for 24h, and immunoblotted for the cytoplasm and nuclear cell lysis NF-κB p65 protein expression. (E) The translocation of NF-κB p65 protein determined by NF-κB p65 staining of NiCl₂-primed BMDMs. Scale bar 50 μm. Data are presented with the means ± standard deviation (n=5). *p < 0.05 and **p < 0.01, compared with the control group.