Research Paper Volume 11, Issue 23 pp 11541—11564
The local translation of KNa in dendritic projections of auditory neurons and the roles of KNa in the transition from hidden to overt hearing loss
Figure 12. Detection of IP3R1, pIP3R1, and its proteolytic fragments in SGNs from WT and DKO cochlea. (A–F) Immunofluorescence detection of the endoplasmic reticulum (ER)-lumen domain of IP3R1(red), phosphorylation site (pIP3R1) (cyan; A–C), N-terminal domain (N-IP3R1) (cyan; C–F). We examined basal cochlear sections at 1.5-mo and 5-mo WT and DKO mice. SGNs were labeled with neuronal marker TuJ1 (blue). The ER was stained with ER-marker (blue). Merged images are shown together with digitally magnified (~3X) images, shown in the last panel. Scale bar 3 μm. For pIP3R1 at 1.5 mos, the percent of SGNs with positive reactivity for WT was 12 ± 3, and DKO was 54 ± 9; (p < 0.0001, data from 7 mice obtained from 25 section/mice). N-IP3R1 at 1.5- and 5-mos, percent cell reactivity for WT = 6 ± 3; DKO = 37 ± 12, and WT = 9 ± 4; DKO = 61 ± 12, respectively (p < 0.0001, data from 6 mice obtained from 25 section/mice). Scale bar = 10 μm.