Research Paper Volume 11, Issue 24 pp 12097—12113

Figure 9. Hydrogen-rich medium increased M2-type macrophage polarization in RAW 264.7 cells after induction, and increased the capture ability of macrophages. (A) Flow cytometry of F4/80, CD206 double-positive macrophages (i.e., M2-type macrophages); (B) Fluorescent staining with ghostpen cyclopeptide on macrophages. n=4 per group. Data are shown as the mean ± SEM. Significance was calculated by one-way ANOVA, *: P<0.05. Abbreviations: Con, control group; LPS/IFN-γ, RAW 264.7 cells treated with LPS and IFN-γ to induce polarization into M1-type macrophages; HRS, RAW 264.7 cells treated with hydrogen-rich medium only; HRS + LPS/IFN-γ, RAW 264.7 cells treated with hydrogen-rich medium after being treated with LPS and IFN-γ.