Figure 4.miR-653 rescues the tumor suppressive effect of ciRs-6 in bladder cancer. (A, B) qPCR was used to detect the levels of ciRs-6, SLC41A2 and miR-653 in each group; (C, D) CCK8 assays were performed to evaluate cell viability; (E–H) clone formation assays were used to evaluate clone forming ability; (I–L) cells in S phase were assessed by cell cycle analysis; (M–O) mouse subcutaneous tumor model experiments were used to detect in vivo tumorigenic effects. Each group contained 5 mice. The results are displayed as the mean±SEM, *p<0.05, **p<0.01.
