Research Paper Volume 11, Issue 23 pp 11136—11147

Figure 3. Ninj2 shRNA or KO inhibits human glioma cell proliferation. A172 glioma cells (A–C, E–G), U251MG glioma cells (D) or the primary human glioma cells (derived two patients, “P1/P2”, D) were transduced with lentiviral Ninj2 shRNAs (“sh-Ninj2”, two different sequences “Seq1/Seq2”), control shRNA (“sh-C”) or the CRISPR/Cas9 Ninj2 KO construct (“ko-Ninj2”), stable cells were established via puromycin selection; Cell proliferation was examined by the assays mentioned in the text (A–D), and cell cycle progression tested by PI-FACS (E and F), with cyclin D1 and cdc2 expression tested and quantified (G). For each assay, n=5. *p<0.05 vs. “Pare” cells. Experiments in this figure were repeated four times, and similar results were obtained.