Research Paper Volume 11, Issue 22 pp 10626—10643

Figure 3. Knockdown of circ_0006332 decreases cell proliferation, colony formation, and the invasiveness of bladder cancer cells. (A) The qRT-PCR analysis shows that bladder cancer cells transfected with siRNA against circ_0006332 significantly reduce the expression of circ_0006332 compared with the controls. Note: siRNA, short interfering RNA against circ_0006332; NC, negative control; siRNA-1, siRNA containing 12 nucleotides from the 5′ end of the siRNA against circ_0006332 and 7 nucleotides from the negative control sequence; siRNA-2, siRNA containing 7 nucleotides from the 3′ end of the siRNA against circ_0006332 and 12 nucleotides from the negative control sequence. (B) CCK-8 assay shows decreased viability of circ_006332-knockdown T24 and UM-UC cells. (C) EdU detection assay shows that circ_006332 knockdown decreases the proportion of cells undergoing DNA synthesis compared with the controls. (D) The graph shows total number of colonies in control and circ_006332-knockdown bladder cancer cells. As shown, circ_006332 knockdown decreases the total number of colonies and the size of the colonies from the bladder cancer cells. (E) Transwell assay results show the total number of cells that invaded the bottom chamber in control and circ_006332-knockdown bladder cancer cell lines in 24 h. As shown, circ_006332 knockdown decreases the invasiveness of bladder cancer cells. Note: All experiments were repeated three times. The data are represented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.