Figure 8. RMF reduced the accumulation of fat and age-related pigments in C. elegans. (A) Representative image of age-related pigments in N2 C. elegans; scale bar = 100 μm. (B) The fluorescence of the fat normalized to tryptophan fluorescence was measured using a microplate reader. (C) The fluorescence of the age-related pigment normalized to tryptophan fluorescence was measured using a microplate reader. (D) Representative image of fat in N2 C. elegans; scale bar = 100 μm. Both experiments were performed on four independent four occasions (days 6, 10 and 14 after the start of RMF treatment). Different letters indicate a significant difference among groups (P < 0.05).