Figure 7. The effects of RMF on ROS and ATP levels and C. elegans physiology. (A) C. elegans food-intake. (B) C. elegans brood size. (C) Total ATP content in C. elegans was detected using a microplate reader. (D, E) The ROS levels in C. elegans were detected with the H2DCF–DA probe by fluorescence microscope and microplate reader.