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Research Paper Volume 11, Issue 20 pp 9075—9089
Sirtuin 3 attenuates neuroinflammation-induced apoptosis in BV-2 microglia
Figure 5. Re-activation of mitochondrial fission abolished the protective effects of Sirt3 on BV-2 cell survival in the presence of LPS. (A) Cellular viability was measured via MTT assay. FCCP was administered to re-activate mitochondrial fission in Sirt3-overexpressing cells. (B) An LDH release assay was used to evaluate cell damage in BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus; FCCP was used to re-activate mitochondrial fission in Sirt3-overexpressing cells. (C, D) TUNEL staining was used to measure cell death after exposure to LPS. FCCP was used to reactivate mitochondrial fission. (E) Cell apoptosis was examined by measuring caspase-3 activity via ELISA. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group; @P<0.05 vs. LPS+adenovirus-Sirt3 group. N=3 independent experiments.