Research Paper Volume 11, Issue 20 pp 8982—8997

Figure 4. Overexpression of HSF1 in the myocardium promotes the expression of Omi mRNA and protein levels by enhancing the activity of the promoter of Omi/HtrA2. Total and nucleoprotein HSF1 expression was increased in aging myocardium, GAPDH, and histone H3 as internal control (A, B). Data are represented as mean +/- SEM. **P< 0.01 vs. young, n = 6 per group. The binding of HSF1 to the Omi/HtrA2 promoter increased in aging mice ©. Total and nucleoprotein HSF1 expression increased after transient transfection of pcDNA3.1-HSF1 (D, E). mRNA and protein levels of Omi/HtrA2 increased after transient transfection of pcDNA3.1-HSF1 (F, G). Relative luciferase activity of PGL-239 increased after transient transfection of pcDNA3.1-HSF1 (H). Values are means ± SEM, *P<0.05, **P<0.01 vs. pcDNA3.1, n = 3. Total and nucleoprotein HSF1 expression decreased after transient transfection of siRNA-HSF1 (I, J). Relative luciferase activity of PGL-239 decreased after transient transfection of siRNA -HSF1 (K). mRNA and protein levels of Omi/HtrA2 increased after transient transfection of pcDNA3.1-HSF1 (L, M), Values are means ± SEM, ^#P<0.05, ^##P<0.01 vs. scramble, n = 3.