Research Paper Volume 11, Issue 20 pp 8969—8981

Figure 2. PCB₁₂₅₄ inhibits the expression of related genes. (A–C) Total RNA was isolated from 661W cells exposed to different concentrations of PCBs for 24 hours. Photoreceptor cell-specific gene (SWS1, CRX, Rho) expression levels were measured by real-time RT-PCR. The results showed that: As PCB concentration increased, RNA Expression levels gradually decreased (statistically significant in the 1 mg/L group). (D) After 24 hours of exposure, total RNA was isolated from the cells and real-time RT-PCR results showed that: expression of miR-20b was increased with increasing concentrations (0.5mg/L group, 1mg/L group was statistically significant), (E–F) Confirmation of miR-20b overexpression and silencing.