Research Paper Volume 11, Issue 19 pp 8418—8432

Figure 2. Inhibiting Sch9 activity by rapamycin or myriocin treatment decreased H₂S production. (A) Diagram showing how rapamycin and myriocin inhibit Sch9 through two different signaling pathways. (B) H₂S production by BY4741 was monitored by using lead acetate strips at 24 or 48 hours after inoculation into YPD medium containing the indicated concentrations of myriocin. (C and D) H₂S production by BY4741 or sulfur assimilatory mutants was monitored by using lead acetate strips which were replaced every 24 hours after the indicated concentrations of rapamycin were added into overnight culture of YPD (* p<0.05 compared to control).