Research Paper Volume 11, Issue 19 pp 8254—8269

Figure 5. The expression of salivary gland functional proteins in primary salivary gland cells (PSGCs). (A, B) The protein and mRNA expression of KLF4 in KL(+/+) PSGCs and KL(-/-) PSGCs. (C–E) Comparison of functional protein or mRNA (aquaporin 5, EGFBP2, ZO-1, and amylase) expression between KL(+/+) PSGCs and KL(-/-) PSGCs by RT-PCR, qRT-PCR, and immunoblot analyses. (F–H) Effects of soluble klotho on functional protein expression. Cells were transfected with pcDNA3-soluble klotho for 48 h. Comparisons of salivary gland functional proteins by RT-PCR, qRT-PCR, and Western blot. The actin protein was used as an internal control. The results are reported as the mean ± SD (*p < 0.05, **p < 0.01).