Figure 2. The expression of COL6A6 and P4HA3 in PA tissues and cell lines. (A and B) RT-qPCR was used to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; (C and D) Immunohistochemistry staining was applied to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; (E and F) Western blotting was used to evaluate the expression of COL6A6 and P4HA3 in PA cell lines. ***P<0.001, compared with normal pituitary tissues; ##P<0.01, compared with AtT-20 cells; ▲▲P<0.01, compared with HP75 cells.