Figure 1. Immunohistochemistry of CACNA1s, CACNB1 and CACNG1 genes. Expression of CACNA1S (A), CACNB1 (B) and CACNG1 (C) in IHC staining finding (X200) increase significantly in old compared to young rats. In western blot analysis of these 3 genes, only CACNA1S is increased in primary cultured lamina propria fibroblasts of old compared to young rats (D). IHC, Immunohistochemistry. One-way ANOVA test; *p<0.05.