Figure 3 — Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

Research Paper|Volume 11, Issue 20|pp. 8745–8759

Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

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Figure 3.lncRNA GAS5 downregulated the expression level of fibrosis-related proteins in MCs. (A–C) mRNA expression levels of FN (A), Col-4 (B), and TGFβ1 (C) in MCs were measured by qPCR. MCs were transfected with LV-GAS5 or sh-GAS5; (D–G) FN, Col-4, and TGFβ1 expression levels were detected by western blot. MCs were transfected with LV-GAS5 and sh-GAS5; (H–J) immunofluorescence analysis showed that FN, Col-4 and TGFβ1 expression levels were downregulated. MCs were transfected with LV-GAS5. *P < 0.05, **P < 0.01.