Figure 2 — Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

Research Paper|Volume 11, Issue 20|pp. 8745–8759

Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

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Figure 2.lncRNA GAS5 alleviated MC proliferation. (A–B) Efficiency of LV-GAS5 and sh-GAS5 were detected by qPCR; (C-D) Proliferating mesangial cells were labeled with EdU. MCs were transfected with LV-GAS5; (E–F) Proliferating mesangial cells were labeled with EdU. MCs were transfected with sh-GAS5; (G–I) Flow cytometric assay showed that GAS5 increased the G0/1 phase of MCs. MCs were transfected with LV-GAS5; (J–K) p53 and p21 expression levels were measured by western blot. MCs were transfected with LV-GAS5. *P < 0.05, **P < 0.01.