This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 16 pp 5975—5991
LINC00511 promotes the malignant phenotype of clear cell renal cell carcinoma by sponging microRNA-625 and thereby increasing cyclin D1 expression
Figure 3. LINC00511 directly sponges miR-625 in ccRCC cells. (A) Nuclear/cytoplasmic fractionation analysis of LINC00511 expression in ccRCC cells. (B) The binding site in LINC00511 for miR-625, as revealed by bioinformatics analysis. The mutant binding sequences in LINC00511 are also shown. (C) A498 and 786-O cells were transfected with the miR-625 mimics or miR-NC. The transfected cells were collected after 48 h incubation and then subjected to RT-qPCR analysis to determine transfection efficiency. *P < 0.05 vs. the miR-NC group. (D) Either LINC00511-Wt or LINC00511-Mut was cotransfected into A498 and 786-O cells with either the miR-625 mimics or miR-NC. After 48 h transfection, the detection of luciferase activity was conducted via a Dual-Luciferase Reporter System. *P < 0.05 vs. the miR-NC group. (E) A RIP assay was conducted to assess the direct interaction between LINC00511 and miR-625. LINC00511 and miR-625 were both immunoprecipitated by the anti-AGO2 antibody from the lysates of A498 and 786-O cells. *P < 0.05 vs. the IgG group. (F) miR-625 expression was quantified in the presence of either si-LINC00511 or si-NC by RT-qPCR. *P < 0.05 vs. the si-NC group. (G) The expression levels of miR-625 in 49 pairs of ccRCC samples and matched adjacent normal renal tissue samples were measured via RT-qPCR. *P < 0.05 vs. normal renal tissues. *P < 0.05 vs. normal renal tissues. (H) An inverse expression correlation between LINC00511 and miR-625 in ccRCC tissue samples was identified by Spearman’s correlation analysis. R2 = 0.3826, P < 0.0001.