Figure 4.LINC00511 promotes the tumorigenesis of OS in nude mice. (A) Xenografts formed by HOS cells with normal (upper) or downregulated (bottom) expression of LINC00511. Tumor tissues were harvested after 3 weeks of implantation (Scale bar, 0.5 cm). (B) Expression level of LINC00511 in the xenografts tissues. *P<0.05 compared with NC group. (C–D) SiRNA-induced downregulation of LINC00511 inhibited the growth of xenografts in nude mice. The volumes of xenografts were measured every 3 days, and their weights were measured after harvesting. (E) Xenografts formed by HOS cells with normal (upper) or downregulated (bottom) expression of LINC00511. Tumor tissues were harvested after 3 weeks of implantation (Scale bar, 0.5 cm). Lenti-sh-LINC00511 cells were transfected with lentivirus-mediated shRNA targeting LINC00511. Lenti-sh-ctrl means cells were transfected with shRNA lentiviral particles with nontargeting scrambled shRNA sequences. (F) The LINC00511 expression level in lenti-sh-LINC00511 group was markedly decreased compared to that in the lenti-sh-ctrl group. (G–H) ShRNA-induced downregulation of LINC00511 inhibited the growth of xenografts in nude mice. The volumes of xenografts were measured every 3 days, and their weights were measured after harvesting. *P<0.05 compared to the lenti-sh-ctrl group.
