Research Paper Volume 11, Issue 14 pp 5035—5057

Figure 8. Western blot analysis confirming the regulatory relationships between KLF5 and its target genes (A) KLF5 expression in pancreatic cancer cell lines and a normal pancreatic cell line. (B) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the HPDE6C7 group. (C) KLF5 expression after transfection of cell with KLF5 siRNA. (D) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the negative control (NC) group. (E) PANC-1 cells were transfected for 48 h with empty vector or KLF5-EGFP plasmid, after which the protein extracts were assayed by western blotting. β-actin was used as a protein loading control. (F) BxPC-3 cells were transfected for 48 h with negative control siRNA, siKLF5-2 or siKLF5-3, after which the protein extracts were assayed by western blotting. β-actin was used as a protein loading control. (G) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the empty vector group. (H) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the NC group.