Figure 2. Relationship between PGP9.5-positive neurons (green) and ICs (red) in the ganglia of rumen MP (A a–i) with age. The neural and IC networks were observed and interweaved with each other in the rumen wall. Higher magnification views of the square frames in (A–c,f,g), the somata and protuberances of two or three ICs, close to each other, formed a ring-boundary layer surrounding each NSs in the ganglia. A decreased density was observed in NS of the ganglia (B), while the MOD of ICs significantly increased in the ganglia (C). (Bar = 20 μm A a–i.)