Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 10 pp 3348—3361
circFADS2 protects LPS-treated chondrocytes from apoptosis acting as an interceptor of miR-498/mTOR cross-talking
Figure 5. circFADS2 targets miR-498. (A) Bioinformatic analysis indicating that miR-498 has a circFADS2 binding site. (B) DLRA was performed following co-transfection of chondrocytes with a luciferase reporter containing either a WT (wild-type) or MU (mutant) circFADS2 and a miR-498 mimic. (C) qPCR analysis showing the decreased miR-498 expression levels in RA patients. (D) Chondrocytes were transfected with si-circFADS2 or si-NC and then treated with LPS. qPCR analysis was used to measure the miR-498 levels in each group. (E) Quantification of miR-498 and GAPDH mRNA by northern blot analysis. *P < 0.05, **P < 0.01 vs. control cells; # P < 0.05 vs. LPS group.