Expression of Concern
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 10 pp 3041—3054
LncRNA HMMR-AS1 promotes proliferation and metastasis of lung adenocarcinoma by regulating MiR-138/sirt6 axis
Figure 6. SIRT6 is a target of miR-138 and is inhibited by HMMR-AS1 knockdown. (A, B) Schematic view of miR-138 putative binding site in the WT/Mut 3′-UTR of SIRT6. Luciferase activity assay in HEK-293T cells transfected with luciferase report plasmids with WT/Mut SIRT6 3′-UTR or miR-138. (C, D) Relative mRNA and protein levels of SIRT6 in NCI-H23 and A549 cells after transfection. (E) SIRT6 mRNA and protein level in NCI-H23 and A549 cells after HMMR-AS1 knockdown. (F) SIRT6 mRNA and protein level in NCI-H23 cells after HMMR-AS1 knockdown and/or miR-138 inhibition. F. Correlation between HMMR-AS1 and SIRT6 expressions in 18 paired LUAD tissues. *P < 0.05, **P < 0.01.