Research Paper Volume 11, Issue 8 pp 2241—2252
Exposure to aristolochic acid I compromises the maturational competency of porcine oocytes via oxidative stress-induced DNA damage
Figure 8. Effects of AAI exposure on the ROS level, DNA damage and early apoptosis in porcine oocytes. (A) Representative images of DCFH staining in control and AAI-exposed oocytes. Scale bar, 40 and 80 μm. (B) The fluorescence intensity of ROS levels was recorded in control and AAI-exposed oocytes. (C) Representative images of DHE staining in control and AAI-exposed oocytes. Scale bar, 20 and 5 μm. (D) The fluorescence intensity of ROS levels was recorded in control and AAI-exposed oocytes. (E) Representative images of DNA damage in control and AAI-exposed oocytes. Scale bar, 5 μm. (F) The fluorescence intensity of γH2AX signals was measured in control and AAI-exposed oocytes. (G) Representative images of apoptotic oocytes in control and AAI-exposed groups. Scale bar, 40 μm. (H) The rate of apoptotic oocytes was recorded in control and AAI-exposed groups. Data in (B), (D), (F) and (H) were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, ***P < 0.001.