Research Paper Volume 11, Issue 2 pp 771—782

Figure 3. EF24 selectively induces apoptosis in SCs. (A) Representative flow cytometric plots of apoptosis assay. WI-38 non-senescent cells (NCs) and IR‐induced senescent cells (IR-SCs) were pretreated with vehicle (Veh) or 10 µM pan-caspase inhibitor Q‐VD‐Oph (QVD), and then treated with 2 µM EF24 for 72 h. Cell apoptosis was assayed by flow cytometer after Annexin-V and PI staining. (B) Percentage of apoptotic (PI^- Annexin V⁺ and PI⁺ Annexin V⁺) NCs. (C) Percentage of apoptotic (PI^- Annexin V⁺ and PI⁺ Annexin V⁺) IR‐SCs. Data are represented as mean ± SEM of three independent assays. ****P < 0.0001.