Research Paper Volume 11, Issue 2 pp 697—706

Figure 2. Concomitant alteration of ERK5 activation with polarization of HG-cultured microglia, which affects the apoptosis of neuronal cells cultured in hypoxic conditions. (A) Western blot for phosphorylated ERK5 (pERK5), compared to the total ERK5 levels in HG- or NG-cultured microglia in different time courses. (B) Western blot for phosphorylated Stat3 (pStat3), compared to the total ERK5 levels in HG- or NG-cultured microglia in different time courses. (C) Conditioned media (CM) from polarized microglia in different conditions and different time courses were added into a neuronal cell line, HCN-2, cultured in hypoxic conditions. (D) TUNEL assay on HCN-2 cells. N=5. *p<0.05.