Figure 3. Age dependent loss of Lin28 in hub cells. (A) Adult testis stained with Lin28 (green) and FasIII (red) in an age dependent manner. Lin28 level, but not FasIII, is decreased in old testis. Scale 10µm. (B) Comparison of the level of intensity of green signal (Lin28 expression) in young (0-1 Day) vs old testis (50 Day). (C) Comparison for the transcript levels of lin28 in young (0-1 Day) and old (40 Days) wildtype testis, showing the reduction in Lin28 levels with age. (D) Hub cell number is not decreased in old testis when Lin28 is overexpressed (upd-Gal4>UAS-lin28) as compared to wild type testis. Testis were stained with Vasa, Traffic Jam (TJ) and FasIII antibodies. Scale 10µm. (E) The number of hub cells in old testis is comparatively reduced in lin28 mutant testis compared to wildtype testis. The number of hub cells in old upd>lin28 (upd-Gal4>UAS-lin28) testis is similar to a young wild type testis (data from