Figure 1. Exercise actions in anxiety-like behavior and resilience to stress in middle-aged female mice at peri-menopause. (A) Over 60% of middle age female mice were in constant estrous. E2 levels were significantly decreased in middle-age females as compared to young females. ***p<0.001 vs young (n=8-9). (B) Middle-aged female mice (9 months old) showed reduced immobility time in the forced swim test compared to young females (2 months old), indicating a lower “depressive-like” state (n=17 per group). (C) Anxiety-like levels measured in the elevated plus maze showed a profound anxiogenic effect of exercise in middle-aged female mice as compared to young mice (n= 5-10). Values of young females were taken from reference