Research Paper Volume 10, Issue 11 pp 3574—3589
Suppression of mTORC1 activity in senescent Ras-transformed cells neither restores autophagy nor abrogates apoptotic death caused by inhibition of MEK/ERK kinases
Figure 7. mTOR inhibition with pp242 uncouples mTOR from the lysosomes. (A) IF images showing pTSC2 (upper panel), mTOR (bottom panel) and lysosome (LAMP1) localization in senescent cells treated with pp242. Arrows show segregation of mTOR and pTSC2 (upper) and mTOR together with lysosomes (lower) in vacuoles and outside of cells. Nuclei stained with DAPI. (B) Western-blot analysis of mTOR levels in senescent cells after 72 h of pp242 treatment. The numbers represent densitometry of the bands.