Figure 2. mTORC1 suppression induces mitochondria damage and an increase of lysosome levels in senescent ERas cells. Mitochondria are stained with Mitotracker Orange, lysosomes are stained with Lysotracker Green and visualized at proper wavelengths. Nuclei were stained with Hoechst33342. Magnification 40x obj + zoom. Graphs below represent intensities of Mitotracker Orange and Lysotracker Green in control and treated cells, measured using ImageJ software.