Research Paper Volume 10, Issue 11 pp 3273—3282

Figure 5. SPC25+ PrC cells present CSC features in vivo. (A-B) Same number of SPC25- or SPC25+ PrC cells from both DU145 and LNCap lines were subcutaneously transplanted into nude mice and assess for the tumor formation by bioluminescence after 8 weeks, shown by representative images (A), and by quantification (B). (C-D) Twenty cells were purified from the tumor developed from implanted SPC25- or SPC25+ cells from DU145 (C) and LNCap (D) lines were subcutaneously transplanted back to new nude mice. A total of 3 rounds of serial adoptive transplantations were performed. The frequency of tumor formation was shown. p<0.05. N=10.