Research Paper Volume 10, Issue 10 pp 2991—3004

Sirt1/Nrf2 pathway is involved in oocyte aging by regulating Cyclin B1

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Figure 2. Cellular distribution of Nrf2 during oocyte meiosis. (A) Immunofluorescent staining was employed to show the subcellular localization of Nrf2. Green, Nrf2; Blue, chromatin. A total of 30 oocytes were examined for each group. Scale bar, 20 μm. (B) Immunofluorescent staining for co-localization of α-tubulin with Nrf2 in mouse oocytes. This shows the co-localization of Nrf2 with spindles in mouse oocyte. Red, Nrf2; green, tubulin; blue, chromatin. Scale bar, 20 μm.