Research Paper Volume 10, Issue 10 pp 2954—2972
Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress
Figure 1. Melatonin attenuates ERS and SASP in cADMSCs. (A) SA-β-gal S of cADMSCs. (P3, 3rd passage, P11, 11th passage, P11–melatonin, melatonin-treated 11th passage) bar = 100 μm. (B) Alizarin Red and alcian blue staining of osteogenic and chondrogenic differentiation of cADMSCs. bar = 50 μm. (C) Immunocytochemistry of γH2AX in cADMSCs. bar = 200 μm. (D) Telomerase activity of cADMSCs. (E) Relative telomere length of cADMSCs. (F) Relative levels of SASP-related transcripts in cADMSCs. (G) Relative levels of ERS-related transcripts in cADMSCs. (H) Western blot quantification of ERS-related proteins (p-PERK and p-IRE1), SASP-related proteins (TNF-a and IL6), and senescent markers (P16 and P21).