Figure 3.USP4 expression significantly impacted HCC cell migration and invasion in vitro. (A) USP4 expression was aberrant in HCC cell lines, as compared to the normal liver cell lines L02 (* P < 0.05, ** P < 0.01). (B) USP4 expression was knocked down by lentivirus technology in SK-Hep1cells (** P < 0.01). (C) USP4 was overexpressed by lentivirus technology in HuH7 cells (*** P < 0.001). (D) Wound-healing assays detected the effect of USP4 knockdown on the healing ability of SK-Hep1 cells (*** P < 0.001). (E) Wound-healing assays detected the effect of USP4 overexpression on the healing ability of HuH7 cells (** P < 0.01, *** P < 0.001). (F) Transwell assays evaluated the effect of USP4 knockdown on the migratory ability of SK-Hep1cells (** P < 0.01). (G) Transwell assays evaluated the effect of USP4 overexpression on the migratory ability of HuH7 cells (** P < 0.01). (H) Matrigel invasion assays examined the effect of USP4 knockdown on the invasive ability of SK-hep1 cells (** P < 0.01). (I) Matrigel invasion assays examined the effect of USP4 overexpression on the invasive ability of HuH7 cells (** P < 0.01).
